Human-induced pluripotent stem cell (h-iPSC)-derived endothelial cells (h-iECs) have become a valuable tool in regenerative medicine. Our group has developed a method for rapid, consistent, and highly efficient generation of h-iECs. Our protocol has broad applications and could reliably provide an unlimited number of h-iECs for vascular therapies.
Key publications
Rapid generation of functional vascular organoids via simultaneous transcription factor activation of endothelial and mural lineages
Gong L, Zhang Y, Zhu Y, Lee U, Luo AC, Li X, Wang X, Chen D, Pu WT, Lin RZ, Ma M, Cui M, Chen K, Wang K *, Melero-Martin JM *
Cell Stem Cell 2025; Jun 13. doi:10.1016/j.stem.2025.05.014. Online ahead of print.
PDF
Robust differentiation of human pluripotent stem cells into mural progenitor cells via transient activation of NKX3.1
Lee U †, Zhang Y †, Zhu Y, Luo AC, Gong L, Tremmel DM, Kim Y, Villarreal VS, Wang X, Lin RZ, Cui M, Ma M, Yuan K, Wang K *, Chen K *, Melero-Martin JM *
Nat Commun 2024;15:8392
PDF
A streamlined method to generate endothelial cells from human pluripotent stem cells via transient doxycycline-inducible ETV2 activation
Luo AC, Wang J, Wang K, Zhu Y, Gong L, Lee U, Li X, Tremmel DM, Lin RZ, Ingber DE, Gorman J, Melero-Martin JM
Angiogenesis 2024;27(4):779-795.
PDF
A comprehensive library of human transcription factors for cell fate engineering
Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal M, Melero-Martin JM, Busskamp V, Church GM
Nat Biotechnol 2021;39(4):510-519
Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA
Wang K, Lin RZ, Hong X, Ng AH, Lee CN, Neumeyer J, Wang G, Wang X, Ma M, Pu WT, Church GM, Melero-Martin JM
Sci Adv 2020;6(30):eaba7606
PDF
Bioengineering hemophilia A-specific microvascular grafts for delivery of full-length factor VIII into the bloodstream
Neumeyer J, Lin RZ, Wang K, Hong X, Hua T, Croteau SE, Neufeld EJ, Melero-Martin JM
Blood Adv 2019;3(24):4166-4176
PDF